SLAS2012 Exhibitor Workshops and Tutorials

Workshops and Tutorials That Help Participants Focus on the Future

Attendees may choose to attend workshops and/or tutorials hosted by SLAS2012 exhibitors. These workshops and tutorials allow conference participants the opportunity to learn about and explore solutions for the unique issues and challenges they need for another year of achievement in laboratory automation and screening. These workshops and tutorials are open to all registered SLAS2012 participants and are presented in the following formats:

Pre-Conference Workshops:

These three-hour workshops are scheduled for Sunday, February 5, 2012 from 9 am - 12 pm. Currently scheduled workshops are:

Creative Use and New Development of Traditional Calcium Influx Imaging Instrument
Sponsored by Hamamatsu

  • Development and screening of red-shifted, genetically encoded calcium indicators protein for in vivo studies.
  • 30Hz and higher data acquisition for calcium oscillation and cardio tox studies.
  • Application of higher throughput of electric field stimulation
  • Studies of light sensitive receptor

Advances in Techniques for GPCR and Ion Channel Screening
Sponsored by Molecular Devices
Join fellow scientists and Molecular Devices experts for a morning of interactive discussions and presentations on novel applications for drug discovery on Molecular Devices instruments. User presented seminars will focus on Ion Channel and GPCR targets, including assays performed with FLIPR® Tetra System and IonWorks™ Barracuda System.

Building the In Vitro to In Vivo Continuum Through Novel, Disruptive Technologies
Sponsored by PerkinElmer
With a growing emphasis on translational insight, it is more important than ever to be able to examine the molecular mechanisms of disease and translate in vitro models into in vivo results. Join colleagues as they explore several state-of-the-art, disruptive technologies that enable simpler, faster results in the research and development of new therapies and drug targets including first in class cell-based epigenetic assays, advanced sequencing sample preparation and data-rich 3D imaging solutions.

Exhibitor Tutorials (90-minute):

These 90-minute sessions are scheduled for Monday and Tuesday, February 6-7, 2012 from 12:30 - 2 pm. Many exhibitors provide refreshments during their sessions.

Monday, February 6, 12:30 - 2:00 pm

Title and Description to be Announced
Sponsored by Beckman Coulter, Inc.

Corning® Epic® Pharmacology — A Label-free Cellular Profiling Service to Enable Biology Driven Lead Prioritization and Optimization
Sponsored by Corning Incorporated
Label-free cellular analysis offers the promise of studying signaling events in endogenous systems, often in highly relevant models systems such as primary cells and stem cells. Our tutorial demonstrates the value of this approach for compound annotation, pathway deconvolution and analysis of off target effects in lead generation activities.

Advances in Cell Lysate Transfer and High-Throughput qPCR Enabled by the Echo® Liquid Handler and Roche Lightcycler®
Sponsored by Labcyte
Quantitative (qPCR) has been demonstrated as a data-rich method for assessing gene expression. Adapting it to a high-throughput method has been challenging due to the required volumes and multiple expression and purification steps. This presentation demonstrates the utility of using the Echo liquid handler from Labcyte, an acoustic, non-contact liquid transfer device, with Roche RealTime ready reagents and the LightCycler® 480 and LightCycler® 1536 to both miniaturize qPCR and simplify the workflow from cell lysate to qPCR data. Cells can be cultured, lysed, and transferred from acoustically qualified, tissue culture treated microplates, into a 384- or 1536-well PCR plate. The Echo liquid handler and the LightCycler® can be incorporated into the Labcyte Access™ workstation, for a walk-away high throughput qPCR solution.

Momentum — Applied to Your Automation
Sponsored by Thermo Fisher Scientific
Thermo Scientific is pleased to host a series of talks by industry leaders and automation managers who present their perspective on the science and performance of Momentum controlled automation platforms. Come and learn how Momentum scheduling software provides an open solution to a range of multivendor automation needs.

Guest speakers will include:
Constantin Radu — Automation Specialist & Laboratory Manager HTS Core Facility, Memorial Sloan Kettering Cancer Centre.
Wolfgang Joerg — Site Engineering and Technology, Boehringer Ingelheim GmbH &Co.
Robert Ries — Leader HTS Automation Support, Boehringer Ingelheim GmbH &Co.
Paul Held — Applications Laboratory Manager, Biotek Instruments, Inc.

Come join us to learn about:

  • Automating and validating science
  • System design considerations and performance goals
  • Criteria for automation software selection
  • Examples of Thermo Scientific integrated solutions
  • Momentum capabilities

Machine Vision Basics and Applications for Lab Automation
Sponsored by MicroScan
Machine vision — the automated extraction of useful information from digital images — is increasingly adopted as an effective means of automating critical processes and increasing laboratory throughput. In this tutorial we cover digital imaging and machine vision basics, as well as typical applications in identification, inspection, measurement and robotic guidance.

Harnessing the Power of Kinetic Image Cytometry to Analyze Fast Biological Events in a High Throughput Manner: Applications for Stem Cell Research in Early Preclinical Toxicology
Sponsored by Vala Sciences
Kinetic Image Cytometry is an emerging high content analysis technique that facilitates cell by cell analysis of fast biological events in a high throughput manner. Join us as we provide and overview of this new technology and demonstrate applications in cardiac stem cell research and early stage preclinical safety testing.

Microfluidic Market: Technologies & Applications Trends
Sponsored by Yole Développement
Microfluidics has entered the Life Sciences market as a key technology to provide solution adapted to the market requirements. Yole Développement follows innovations and key players in this market for several years. This is a fast evolving market, starting its consolidation phase. Combined analysis of the technologies, key players, applications and requirements is essential to understand this market dynamics.

Tuesday, February 7, 12:30 - 2 pm

Fluorescence Lifetime Technology in Screening and Profiling
Sponsored by Almac Group Ltd.
Fluorescence Lifetime technology (FLT) is a unique reading modality that provides a cost effective yet powerful and robust solution to screening that avoids the problems of compound interference whilst allowing profiling groups access to a wide range of assays catering for difficult to assay targets. FLT users from Large Pharma screening groups and CROs will relate their experiences as well as reagent and instrumentation providers.

Innovative multiparameter HT screening: Faster, cheaper, better
Sponsored by Beckman Coulter, Inc.
High-throughput flow cytometry (HTFC) with novel cellular models such as stem cells, and automated result generation can revolutionize screening. Full automation is possible and necessary, increasing throughput, assay robustness, and reproducibility. The current image-based HCS pipeline will be compared with multiparametric HTFC demonstrating the power of HTFC in screening regimens.

HTRF® Assays in Daily Lab Practice: The Opinion From Industry and Academic Experts
Sponsored by CisBio Bioassays
This tutorial will give the opportunity to scientists and experts from the industry and academia to share their practice of multiple HTRF® assays, such as receptor ligand binding, functional biomarkers or target specific assays, in their daily screening operations and research.

Increasing the Power and Scope of Phenotypic Screening With the IntelliCyt HTFC® Screening System
Sponsored by IntelliCyt Corporation
The IntelliCyt HTFC Screening System is a powerful new tool for phenotypic screening using suspension assays. This platform enables robust screens to evaluate compound effects on nonadherent cell lines, primary immune system cells and molecular interactions using bead based assays. The HTFC Screening System completes the phenotypic screening toolbox.

Miniaturized Assays using the Echo® liquid handler
Sponsored by Labcyte
The Echo liquid handler uses acoustic energy to transfer fluids from microplate to microplate, in a completely tipless, non-contact manner. Transfer precision and accuracy provides robust results even with the reduced volume scale. We demonstrate the utility in several assays including the GloSensor™ cAMP assay from Promega. Results are consistent with experimental data from larger-scale formats, with consistent precision and accuracy even in higher density and lower volume formats.

Promega/Gilson Product Demonstration
Sponsored by Promega Corporation and Gilson
Room: SDCC, Room 14B, Mezzanine Level
Benchtop Automation Product Demonstration.

Exhibitor Tutorials (50-minute):

These 50-minute sessions are scheduled for Monday, February 6, 2012, 12:30 - 1:20 pm, and 2 - 2:50 pm and Tuesday, February 7, 2012 from 8 - 8:50 am, 12:30 - 1:20pm, and 2 - 2:50 pm. Many exhibitors provide refreshments during their sessions.

Monday, February 6, 12:30 - 1:20 pm

High-Throughput, Label-Free Screening of Intractable Targets Using RapidFire Mass Spectrometry
Sponsored by Agilent
Agilent's RapidFire Mass Spec system provides researchers with an important competitive advantage in the search for new lead candidates. In this seminar, we'll present data and case studies to help you learn how:

  • To accelerate label-free HTS
  • To enable target classes
  • RapidFire technology enables functional biochemical assays

Effect of Liquid Handling Quality Control on Biological Assay Performance
Sponsored by Artel
In order to obtain promising HTS candidates, high quality assays are required. Assay variability is a combination of liquid handling, biological and random variability. Liquid handling variability is often underappreciated, yet can have a huge impact on the outcome of an assay. This tutorial will demonstrate how an improperly calibrated liquid handler can affect the performance of a model biological assay.

Advancing the Evaluation of Cell-Mediated Cytotoxicity Through the Use of an Automated Luminescent ADCC Assay
Sponsored by BioTek Instruments, Inc.
The success of biologic therapeutics has begun to reshape today's pharmaceutical market. Antibody-dependent cell-mediated cytotoxicity (ADCC) assays are a central method for assessing biotherapeutics. In this tutorial we will describe the use of a non-radioactive luminescent chemistry to simplify the assay process and provide improved data quality, relative to the currently used radioactive 51Cr procedure. The assay is easily automated in 96-well format to further simplify and create a more robust process. Experimental data using control antibodies, including Rituximab, as well as fresh and cryopreserved NK cells will be shown.

CHOICE™ Technology — New Level of Flexible and Highly Parallel Pipetting
Sponsored by CyBio
CyBio is proud to unveil its latest Liquid Handling scalable platform, CyBi®-FeliX, this year's NPA candidate. The remarkable wide volume range and format flexibility based on the innovative, patent pending CHOICETM Technology set a new standard for benchtop pipettors. 12 deck positions on less than 26" x 18" (W/D) footprint define a never before seen level of compactness. The novel platform addresses a wide set of applications in the field of proteomics and genomics as well as biological and biochemical area.

Modernizing Workflows Using Higher Throughput, Micro-Fluidic Free and Label-Free Protein Binding Assays
Sponsored by ForteBio
Bio-layer interferometry utilizes a fiber optic based biosensor to probe protein-protein and small molecule-protein binding events in the well of a standard 96- or 384-well microtiter plate. The plate based assay enables Dip and Read format of up to 16 parallel and fully independent biosensors without microfluidics. Molecular binding data may be used for both quantitation and kinetic applications. For quantitation, analyte concentrations are measured directly in crude supernatants or lysates with read times as fast as15 minutes with no sample preparation. Real-time kinetic data allow kon, koff and KD as well as screening applications, such as epitope binning, off-rate screening and antibody pair selection. Example applications and the efficiency gains achieved by industry implemented workflows will be discussed.

Monday, February 6, 2 - 2:50 pm

Screening Epigenetic Targets with the Transcreener® HTS Assay Platform
Sponsored by BellBrook Labs, Inc.
Enzymes responsible for epigenetic modifications to chromatin are the focus of intense drug discovery efforts for cancer and other diseases. We will describe how the Transcreener® HTS Assay platform can be used to screen diverse epigenetic modifying enzymes including histone and DNA methyltransferases, acetyltransferases, glycosyltransferases, kinases, and SUMO and ubiquitin ligases.

Biotix Tips vs. OEM: Case Scenarios for Compounds Transfer and for Ca++ Signaling Assays on the FLIPR Tetra
Sponsored by Biotix
This tutorial will provide an in-depth examination of key considerations for laboratories working to develop and implement miniaturized screens onto automated screening platforms. Tim Spicer, M.S., a senior scientist in the Department of Lead Identification at The Scripps Research Institute will discuss their experience in evaluating Biotix robotic tips against OEM products for compounds transfer and Ca++ signaling assays on the FLIPR Tetra system.

Drug Discovery and Screening Reagents and Assay Kits From Cytoskeleton, Inc.
Sponsored by Cytoskeleton, Inc.
Cytoskeleton, Inc. provides reagents for use in drug discovery and screening for neurobiology, inflammation, hypertension, microbiology and cancer-focused projects. We offer high quality small G-proteins, actins, tubulins, FtsZ and motor proteins along with kits to measure their functional activity. Stop by our tutorial on the Mezzanine level to learn more.

Analysis of Cellular Signaling in Cell-Based Assays Using Post-Translational Modification State-Specific Antibodies
Sponsored by Cell Signaling Technology
Speaker: Roberto Campos-Gonzalez
Post-translational modification state-specific antibodies (e.g., phospho-specific, acetyl-specific) allow quantitative analysis of signaling in cell-based assays, and are useful to monitor expression of a specific protein or activity of a specific pathway. We will discuss fixation, permeabilization, and staining protocols optimized for signaling antibodies, including our premium XP® monoclonal antibodies.

Investigating Next Generation Receptor Biology Using PathHunter®: Application to Receptor Trafficking and Ion Channel Activation
Sponsored by DiscoveRx Corporation
The PathHunter® ß-galactosidase based complementation system has been widely employed to monitor protein interactions and translocation in a cell-based, screening friendly format. Recently we have adapted this technology to measuring receptor internalization and trafficking through the secretory pathway. Here we present the application of this method to ion channel activation and the detection of pharmacochaperone activity.

Genedata Screener, the First Choice for Integration and Analysis of High Content Data, Adds Tools for Cell-Level Data Analysis and Condensing
Sponsored by Genedata
Screener leads in HCS by loading plate data and images from all high content readers and databases. Now Screener has added tools and interfaces to support interactive analysis and condensing of per-well populations of cell data. This tutorial will review Screener's core capabilities and introduce this exciting new functionality.

High Content Imaging Assays on the ImageXpress® Micro XL HCS System
Sponsored by Molecular Devices
Learn how the ImageXpress® Micro XL HCS system can accelerate your discovery by doubling by doubling the throughput of your imaging assays. We will present three examples; a high resolution, GPCR activation assay with improved performance, a neuronal toxicity assay with improved statistics and a 3 color cytotoxicity assay that was accelerated to assess greater than 10 million cells/hour.

Optimizing Reproducibility and Physiological Relevance of Primary and Stem Cell Research Through the Automation of Label-Free Analysis
Sponsored by PerkinElmer
GPCRs and ion channels are two of the most important drug discovery target classes, and are frequently assayed in recombinant systems measuring the modulation of specific cellular biomarkers associated with receptor activation. There is a growing demand, however, for more physiologically-relevant assay platforms, leading to increased adoption of both label-free technologies and stem cells. This tutorial discusses how the automation of label-free technology allows non invasive analysis of primary and stem cells in a more efficient and predictive way.

ONE-Glo™+Tox: A Multiplexed Assay to Monitor Reporter Gene Expression in the Context of Cell Health
Sponsored by Promega
Reporter gene assays are sensitive indicators of cell signaling events. Without a proper viability control, the interpretation of data can be skewed if reporter response is affected by changes in cell health. We present a multiplex approach combing a non-lytic cell viability assay with a robust firefly luciferase assay.

Optimiser Microfluidic Microplates Enable Unparalleled Flexibility in ELISA
Sponsored by Siloam Biosciences
Optimiser-based ELISA's can be tailored towards small sample volume, rapid assay time, and ultra-high sensitivity. Using a 5 microliter sample, Optimiser ELISAs can achieve 1pg/mL sensitivity in a 2 hour assay. By repeating the sample loading, sensitivity can be amplified 1000fold in a 3.5 hour assay.

Tuesday, February 7, 8 - 8:50 am

Transcreener® HTS Enzyme Assay Development Service
Sponsored by BellBrook Labs, Inc.
As drug discovery efforts transition to more diverse and less extensively studied enzymes, assay development becomes more challenging and expensive. Transcreener HTS Assays use homogenous, fluorescent detection of nucleotides to enable detection of thousands of enzymes with just a few assays. We will describe how the Transcreener HTS Enzyme Assay Development Service can help you move challenging targets into screens more rapidly, reduce your assay development costs, and insure high quality screening data, with case studies on GTPases, methyltransferases and glycosyltransferases.

Epigenetics Drug Discovery: Success Delivered by BioFocus
Sponsored by BioFocus
BioFocus has identified a number of novel chemotypes leading into further optimization. The epigenetics drug discovery tool-box is continuously expanded:

  • Design of efficient and selective inhibitors of epigenetics readers and writers using proprietary chemogenomics tools.
  • Profiling strategies for lead optimization of epigenetics targets family inhibitors.

Automated Platform Tailored for HTS in Nanoliter Volumes- The Ability to Go Smaller
Sponsored by BMG LabTech and Labcyte
As 1536-well plates were introduced to high throughput screening (HTS), adoption was not immediate. Now instruments with 1536-well capability are a mandatory consideration for most HTS workflows. Will the pattern stay true for screening in 3456-well formats? In this tutorial, BMG LABTECH and Labcyte present a new platform incorporating next generation instrumentation and software for nanoliter-scale HTS. See how this fully automated nanoHTS platform can screen more compounds faster while lowering your average screening costs.

Automated Biorepository Sample Workflow
Sponsored by Brooks Life Science Systems
New biobanks are being set up around the World; automated sample processing is improving processing speed and confidence in sample tracking. This tutorial considers a number of biobanking workflows, focusing on interactions between the sample containers and automation. A case study is presented of the fully automated workflow at the Aurora Healthcare Orbit Biobank.

Novel Assays for Drug Discovery and Toxicology Using Human iPS Cell-Derived Neurons and Cardiomyocytes
Sponsored by Cellular Dynamics and Molecular Devices
Biopharma continues to adapt cell-based assays for primary/secondary screening to reduce late-stage compound attrition. Human cells derived from induced pluripotent stem cells (iPSCs) overcome existing models' limitations in functionality, purity and reproducibility and offer an unlimited supply of well-characterized cells for drug efficacy screening and safety testing. This presentation will demonstrate the utility of using iCell® Cardiomycytes and Neurons in conjunction with the ImageXpress®Micro High Content Screening System and FLIPR® Tetra System.

Tips and Best Practices for Improving Your Biochemical and Cell Based Assay Performance
Sponsored by Corning Incorporated
Today's scientists rely heavily on in vitro assays, both biochemical and cell-based in their research. Critical parameters in achieving success in these assays include understanding the assay dynamics, the instrumentation involved in reading the assay and in the case of cell-based assays understanding how the environment in which the cells are grown impact cellular physiology. Such factors as microplate geometry, density, surfaces and instrumentation all play an important roles in the success of the assay. In this tutorial we will discuss ways to enhance your assay performance. Topics to be discussed include:

  • Understanding your choices of microplates and their roles in assay success
  • Discovering the varieties of microplate coatings and surfaces for your assay
  • Important considerations in cell-based assays
  • The importance of choosing the correct instrumentation and settings to run your assay

New Trends in Laboratory Automation
Sponsored by Hamilton Company
As automation liquid handling continues to evolve and radically advance life science research, so do the challenges associated with the latest trends in automation. Join this informative Hamilton Robotics and Hamilton Storage Technologies' workshop to learn about our new products and how our innovative solutions for automated liquid handling and sample storage serve the current market needs.

Tecan Launches Infinite® M 1000 PRO With AlphaScreen®/AlphaLISA®
Sponsored by Tecan
This tutorial features the new Infinite M1000 PRO monochromator based multimode reader with its new module for AlphaScreen/AlphaLISA measurements and an ingenious temperature correction function to compensate for sample temperature variations. Apart from adjustable fluorescence bandwidth, FRET, TR-FRET and FP, the instrument allows Tecan's NanoQuant Plate™ compatibility, improved injectors, absorbance, fluorescence, 3D and luminescence scanning. AlphaScreen and AlphaLISA are registered trademarks of PerkinElmer, USA.

Linear Bar Coding and Sample Tracking
Sponsored by Thermo Fisher Scientific
A bar code is a machine-readable code which is used when samples are to be processed, analyzed or stored. Bar coding simplifies your tracking and inventory management establishing historical identification of contents at any point in an assay, method or tracking of samples.

Tuesday, February 7, 12:30 - 1:20 pm

Analytical Scale Protein Sample Preparation and Assay: Rapid ELISAs and Fully Automated Peptide Mapping Workflows on the AssayMAP Bravo Platform
Sponsored by Agilent
The Agilent AssayMAP Bravo platform offers automated sample preparation for biological characterization workflows such as Peptide Mapping and assay solutions such as rapid ELISAs. The platform consists of precise positive displacement flow control by the 96 channel Bravo liquid handler with disposable 5µL cartridges, packed with any of the various chromatographic supports.

New HTS Tools for Discovering Low Affinity Inhibitors in Primary and Secondary Screens
Sponsored by BMG LabTech
Analysis of high-throughput screening data has shown two trends, classic HTS platforms have not delivered the number of expected drug leads as once thought and the more promising leads have lower molecular weights. Since HTS assays are performed at low volumes and low concentrations, are the more marketable lower affinity drug targets being missed with current, less sensitive platforms? In this tutorial learn how lower affinity hits are found in a primary and a secondary high-throughput screen that were missed using current technology. The next generation PHERAstar FS outperforms two competing drug discovery technologies, a charged coupled device (CCD) based microplate reader used for HTS and a flow cytometer used for high content screening (HCS). Being faster and having far superior assay quality parameters (Z prime, delta F% and assay window) in the IP-One HTRF® and fluorescent cell-based assays, the PHERAstar FS represents a new choice for finding low affinity compounds in primary and secondary screening paradigms.

New Tools for Discovering Low Affinity Inhibitors of the Inositol 1,4,5-triphosphate (IP3) Signaling Pathway
Sponsored by BMG LABTECH
Analysis of high-throughput screening data has shown two trends, classic HTS platforms have not delivered the number of expected drug leads as once thought and the more promising lead compounds have low molecular weights (‹500 Da). Since classic HTS platforms are performed at low volumes and low concentrations, are the more marketable lower affinity drug targets being missed with older, less sensitive platforms? In this tutorial learn how when using the IP-One HTRF® assay from Cisbio, lower affinity hits were found on the next generation PHERAstar FS microplate reader but were completely missed on a leading CCD based reader. Being more than twice as fast and having far superior assay quality parameters (Z prime, delta F% and assay window), the PHERAstar FS represents a new choice for finding low affinity compounds in this and other HTS screening paradigms.

Assaying the Multiple Energy-Producing Pathways of Mammalian Cells Using Biolog's PMM/OmniLog Automated Platform
Sponsored by Biolog, Inc.
To elucidate metabolic changes that occur in cell-based assays, diabetes, obesity, cancer, cell activation and differentiation, it is important to understand multiple cellular energy metabolism pathways, mitochondria and their alterations. Biolog's PMM cell-based assays can be employed as a fundamental tool in genotype-phenotype studies to determine metabolic changes in cells due to chemical agents, genetic regulation, epigenetic changes, differentiation or mutation.

CyBio® Scheduler — Biology Meets Throughput — Realizing Complex Assay Systems in an Ultra High-Throughput (uHTS) setting
Sponsored by CyBio
Continuing the tradition of presenting the real life use of CyBio® Scheduler, CyBio is pleased to present a workshop geared to interested customers and power users alike. The advantage of special features like the constant cycle repetition or the guaranteed avoidance of deadlocks in the CyBio® Scheduler will be described. A user report will give you a perspective of the practical use of these features in responding to daily challenges.

Label-Free Assays for Protein Quantitation and Binding Kinetics in a Drop of Sample
Sponsored by ForteBio
Bio-layer interferometry (BLI) has gained popularity in recent years as a reliable, yet simple method for analyzing protein-protein and other biomolecular interactions. BLI has the advantage of probing unmodified proteins, thus eliminating potential perturbation of the interactions being studied, and, avoiding the time-consuming and expensive steps required by the use of labels. To further enhance the ease with which label-free analysis can be performed, we have developed new methods using BLI that allow for rapid identification, quantitation and characterization of expressed protein from a very low volume of sample. New methods and data will be presented to illustrate the ease of incorporating BLI into a lab workflow.

Accelerated Cell-Based Screening Using MaxCyte Large Volume Transient Transfection: MaxCyte Synergy With Sophion Q-Patch and Codex ACTOne High-Throughput Assays
Sponsored by MaxCyte
The addition of the MaxCyte STX Scalable Transfection System to screening and profiling pipelines produces quality assay results without the need to create stable cell lines thus enabling quicker turn-around from project initiation to data generation. The broad cell type compatibility and reproducibility of MaxCyte transient transfection provide for integration with the latest in cellular screening assays for a variety of targets including ion channels, GPCRs, kinases and nuclear receptors. In this tutorial MaxCyte will present data for large volume transfection of cells using the MaxCyte STX as well as performance data of the transfected cells using Sophion's QPatch automated patch clamp system and Codex BioSolution's ACTOne Technology for GPCR screening.

Three Ways to Successfully Implement a Label Free High-Throughput LC-MS Assay
Sponsored by Thermo Fisher Scientific
Improvements in LC and MS components have enabled the rapid and specific analysis of a wide range of sample types. These improvements have allowed LC-MS detection in areas previously reserved for plate reader based technologies. This discussion covers LC-MS label-free benefits and tips to implementing high-throughput LC-MS assays.

Tuesday, February 7, 2 - 2:50 pm

Drug Discovery With Discriminating Fluorescence Lifetime Technologies
Sponsored by Assaymetrics Limited
Fluorescence lifetime technologies are now effective tools for drug discovery and development. Instruments are now sufficiently fast for high-throughput screening and can be integrated into existing robotics systems. Simple, robust kinase and protease fluorescence lifetime assays can be miniaturised to 3uL in 1536 plates for cost-effective profiling and screening.

Get InSPRed by ProteOn and Accelerate Your Label Free Interaction Analysis
Sponsored by Bio Rad Laboratories
The ProteOn protein interaction array system is a multiplex label free biosensor capable of measuring 36 interactions simultaneously. Attend this tutorial to learn how the ProteOn is accelerating drug discovery in both Parma and academic laboratories. Imagine the possibilities with multiplexed SPR.

Characterizing Human iPSC-derived Neuronal Cultures: Expression and Function of ion Channels and Receptors
Sponsored by Cellular Dynamics International With Actelion
Human iPSC-derived neurons offer unprecedented opportunities for drug discovery and development. Here Actelion characterizes electrophysiological properties of CDI's iCell® Neurons and compare expression and function of key ion channels and receptors in these iPSC-derived neurons vs. rodent and human primary neuronal cultures at different culture ages.

Biochemical and Aggregation Screening Using Corning® Epic® Label-Free Technology
Sponsored by Corning Incorporated
Learn how to utilize Corning Epic Label-Free Technology for high-throughput biochemical screening. Listen to industry experts on how to eliminate false positive hits coming from aggregation.

KINOMEscan™: Ideal Platform for Kinase HTS, Lead Op and Beyond
Sponsored by DiscoveRx Corporation
Despite some high-profile successes, the tendency of SMKIs to interact with multiple kinases presents many challenges and opportunities. This tutorial will provide a technology overview, case studies illustrating how KINOMEscan™ can support all stages of drug discovery and optimization, plus a look at how the technology is being applied to novel target classes.

To Affinity and Beyond: Novel Approaches to Fragment Screening and Lead Characterization With SPR-Based Biosensors
Sponsored by GE Healthcare
SPR-based biosensors are becoming a powerful tool in lead finding and drug discovery due to the sensitivity requirements to observe binding interactions of low-affinity and low-molecular weight compounds to target proteins. This offers not only novel ways of hit finding, but also more efficient ways to identify and advance true hits for chemistry and later stage biology by providing quantitative binding information for ranking fragments by affinity and ligand efficiency. This information is critical to support ongoing structure–activity efforts during fragment hit-to-lead development. In this workshop, the basic strategy of the technology used, key applications in screening/lead finding projects, and general best practices in working with small molecules and fragments will be detailed and discussed.

Time-Dependent Data From Kinetics, Label-Free, and Ion-Channel Experiments Can all be Interactively Analyzed With Genedata Screener
Sponsored by Genedata
Working with time-dependent data can be difficult due to the inability to see individual traces, inflexibility with instrument-defined analysis methods, and dealing with data from different technologies. This tutorial shows how Screener solves all these issues, loads all data types, and provides advanced user interactivity for custom analysis and visualization.

High-Content Analysis of Signaling Networks Using NodeSensor™ Assays
Sponsored by Lonza
To analyze signaling pathways, using our NodeSensor™ assays, each protein partner is fused to one fragment of a rationally dissected reporter protein, and both are co-expressed in a living cell. Only when the two interacting proteins form a complex, the reporter protein fragments can re-fold and be detected by HCA.

The Next Generation in Microbial Colony Picking: Introduce Fluorescent Capability to Your Workflow
Sponsored by Molecular Devices
Molecular Devices is pleased to announce the latest technology in flurescent micobial colony picking. Building on the success of our Qpix range we have developed a new family of microbial colony pickers to meet the needs of tomorrow's applications. Notably our new range of microbial colony pickers facilitate both white light and fluorescent imaging. This workshop will highlight these new systems.

NanoLuc: A Smaller, Brighter, and More Stable Luciferase Reporter
Sponsored by Promega
Promega scientists have engineered a novel luciferase and corresponding assay chemistry with significant improvement over current technologies. The new luciferase — NanoLuc — is smaller (~1/3) and brighter (~100-fold), and also exhibits significant physical stability. Combined with a new assay reagent — NanoGlo — that elicits ATP-independent luminescence, the new reporter technology can supply ample luminescence in challenging biological systems.

Direct Digital Dispense Technology Enables Faster, More Accurate Drug Screening
Sponsored by Tecan
Serial dilution is ubiquitous in preclinical discovery, used for dose-response analyses from assay development to lead optimization, ADMET and drug-drug interaction studies. With HP Direct Digital Dispense technology, titrations are performed at the bench top without the need for intermediate dilutions. Volumes ranging from 13 pL to 5 uL are dispensed into each well to independently generate each required dose, providing any dose in any well.